IHC 13th gw

The sample №1 (60mm) is from the old part of the Collection, thus immunoreactivity could be rather artifitial. For a good-quality immunoreactive preparations and comparison see additional sections from other Collection specimens.

#GFAP – immunonegative 

#vimentin (Vim)- In the presumptive 11th neocortex, Vim-immunoreactivity was demonstrated in ventricular (VZ) and subventricular (SVZ) zones ( #36, #61 ). In the ventricular zone, an intense and clear IHC reaction with antibodies to vimentin was visible in the cell bodies adjacent to the surface of the lateral ventricles and in their radially directed processes. At the border of the ventricular and subventricular zones, a clear radial striation was disturbed and mainly multidirectional processes of cells were found. In the other zones (intermediate zone, subplate, cortical plate and marginal zone) the reaction to vimentin was less pronounced, while it was detected in the wall of relatively large blood vessels. Thus, the intensity of the reaction to vimentin in the cells of the nervous system and their processes was decreased from the ventricular to the marginal zone.

At the 11-12th gw stage, in this specimen #NeuN-immunoreactive cells were found in all transient zones of the hemispheric wall (#85). However, the Neun+ cell density was unequal across the zones: the greatest density was found in the ventricular zone and in the differentiating cortical plate, while in the intermediate there was a low density. Interestingly, in the subplate zone, the density of the labeled nuclei distribution was higher than in the cortical plate. In the marginal zone, the subpial granular layer was well determined with the help of this marker. 

See additional specimens for NeuN-immunoreactivity pattern in the 10-11th gw stage